Only one 0- 100 psi analog gauge is supplied with this number, and it is recommended to operate with two gauges, one for the feed and one for the retentate. Converge, Intensify and Evolve. To relieve hydraulic system pressure at any time, set the System Mode Selector Switch to DUMP or open the System Pressure Manual Dump Valve, MV-1 on the left side of the Hydraulic Power Unit. Set the torque wrench at 180 . This guide provides installation and maintenance procedures for Pellicon 3 cassettes. Use a few drops of suitable lubricant on the tie-rods and between the nuts and the washers, whenever permitted. Temperature change since last time holder was torqued. Screen Type. This guide provides installation and maintenance procedures for Pellicon 3 cassettes. endstream endobj 259 0 obj <>stream HyTSwoc [5laQIBHADED2mtFOE.c}088GNg9w '0 Jb The consent submitted will only be used for data processing originating from this website. 0000003603 00000 n Pellicon 3 Cassettes with Ultracel Membrane Performance Guide Longer exposure may result in membrane damage. .. . .. .. . . . .. .. R`ms numbc prevmbcs mgsthaahtmeg hgb epcrhtmeg prelcburcs jer Tcaamleg, Lcrtmjmlhtc ej Xuhamty suppamcb wmt` chl` lhsscttc jer, feacluahr wcmn`t amfmts. endstream endobj 267 0 obj <>stream The acceptance criterion for cleaning efficacy as measured by NWP is membrane and application specific, and may vary between plants. Continue with Recommended Cookies, Home Millipore Millipore Pellicon 3 Cassettes with Ultracel Installation Guide. Inspect the tie rods and nuts for signs of burrs or stripped threads. (20.3 to 22.6 Nm) by holding the spring loaded lock collar down and turning the handle until the 180 to 200 in. Find the products and services for your process. Pellicon 3 88 cm 2 cassette filter holder | Sigma-Aldrich It will maintain appropriate compression on the cassettes during processing or during storage of installed filter cassettes. 2 3 Pellicon 2 Cassettes Installation, User, and Maintenance Guide 16. hbbd``b`a: "TA,M b "LHpE @b#c@ o 0000031202 00000 n For the low-retentate volume holder, order the fitting kit catalog number XX42PK160. Pellicon Single-Pass TFF Installation Pellicon 2 Mini Cassette Installation Required Equipment Check thread for nicks or dents; if found replace tie rod or nuts. Find the products and services for your process. A mild solvent such as IPA and water is generally effective. Pellicon Stainless Steel Holder | Sigma-Aldrich User Guides. The filters may also be removed from the system and placed in sealed bags or containers. Trust in one of the most highly published & validated portfolios of antibodies. User Guides. Attach the socket to the torque wrench by firmly pressing the socket onto the torque wrench drive. Hydrophobic and hydrophilic membranes for filtration and venting applications. If your wrench has a ratchet style head, move the lever to the right lock position to tighten the nuts or to the left lock position to loosen the nuts. Water for injection should be used during depyrogenation. Visually inspect filter, replace as needed. This Website requires your browser to be JavaScript enabled. Assemble the TFF system as shown in Figure 2. Greater control & increased flexibility for both small & large molecule development & manufacturing processes. lhg `cap bctcrfmgc w`ht fcforhgc rhtmgn ms hppamlhoac jer h phrtmluahr prelcss. 1. Slide the end plate with the handle side up onto the tie rods. Pellicon 3 Cassette with Ultracel 30 kDa Membrane, C screen, 0.11 m - Find MSDS or SDS, a COA, data sheets and more information. The Pellicon XL 50 allows process development and scaling studies. Fully open the retentate valve (V4) (and permeate valve (V5) if present) and direct both the retentate and filtrate to the tank. Assemble the TFF system as shown in Figure 2. Maintain clean baselines and improve chromatography run reproducibility with efficient filtration. Replace the washers and nuts. &T](l%1m%Wwv&RK=\C/qpzqdJVB bm@;,=d,C^}`V,B 0000002183 00000 n Slide additional Pellicon 3 Cassettes onto the holder if more membrane area is required. Membrane Research. Our Pellicon family of filtration products are the ideal solution for a range of high value applications. Pellicon3 Cassettes - Installation and User Guide. endstream endobj 268 0 obj <>stream Pellicon 3 Cassette with Ultracel 30 kDa Membrane, C screen, 88 cm Tighten the nuts using the torque wrench and 15/16 in. 2. Measurement of Normalized Water Permeability. 2. endstream endobj 261 0 obj <>stream Slide the end plate onto the tie rods and press the end plate against the cassette. It appears that your browser has JavaScript disabled. Pellicon 3 Cassette with Ultracel Membrane filtration area 88 cm2 14. The normalized water permeability (NWP) for Pellicon 3 Cassettes should be established prior to the first product contact of each filter. It is not intended to provide validation protocols or supporting data for validation purposes. These products are manufactured in a facility which adheres to FDA Good Manufacturing Practices. Slide the manifold adapter plate directly up against the stainless steel manifold. 0000016437 00000 n 80 psi TMP at 4-40C; 40 psi TMP at 4-50C. Pellicon3 Cassettes - Installation and User Guide. US EN. Pellicon 3 Cassettes Installation and User Guide 1 3. Converge, Intensify and Evolve. XX42P0060 Pellicon Cassette Acrylic Filter Holder, XX42PRV60 Pellicon Cassette Acrylic Holder Low Retentate Volume, XX42P0080 Pellicon Cassette Stainless Steel Holder, XX42P0K80 Pellicon Cassette Stainless Steel Holder Assembly. At the higher concentrations, total exposure time of Pellicon 3 Cassettes with Ultracel membrane must not exceed 100 hours and total exposure time of Pellicon 3 Cassettes with Biomax membrane must not exceed 200 hours. HlK\!Dwl!aYCK%_JH=6O2M5#r(D}Xjzhu%=el>PgLY.0`lnD Olu%q-gS>2P9@QbyYyn. endstream endobj 264 0 obj <>stream 170 mL in feed channel. Pioneering excellence with Steritest Symbio Pumps. 30 psi at 25C for 24 hours. Recirculate the water for 10 minutes. Place enough spacers on each rod so that the length of exposed threaded tie rod is less than the length of the hex socket. hgb Lhghbh. ',Av]g_M&Ug3pZe3Op%]!i@_#(%F7~=0|:K )RBw_/G}nP%igyuWtXU#7{ 8t}pnP%m&f`HnAd{9{0DT8 oSt4mx xiTtlSC8)9T"_#>)4iw}PII-\Kg6mqF^?atrw6R@HhB.:Nv/u9--_|m~EYx~B#!%K>c Um7[UrR7Vt*Jmh_S:t:uZN7i1mfdntjUSnre&|>x^0{ U .{ }9g5!-_-gn. However, a chemical sanitization can be done; refer to the user guide for recommendations. Type C screen (coarse screen) Maximum Operating Temperature and Pressure. Your fast track through regulatory challenges.. Functional excipients to enhance bioavailability. What Generation is Your Sterility Testing Pump? At the end of the recirculation, fully open the retentate and filtrate lines, turn off the feed pump, and drain the retentate and filtrate piping. endstream endobj 262 0 obj <>stream See remedy under Leak Between Filters above. Loosen and remove the hand wheels on the clamp rods at the opposite end plate (of the same holder level). Pellicon 3 Cassette with Ultracel 30 kDa Membrane, C screen, 1.14 m 2. 0000016173 00000 n Permeate line must be completey emptied before measuring air flow. Properties material TPE seal composite regenerated cellulose (CRC) membrane polypropylene polypropylene cap (for end) polypropylene screen Quality Level 400 feature Manifold adapter plate for Pellicon 3 cassette manual process-scale holder and benchtop holder installation. cL> The membranes for Pellicon 3 cassettes are not autoclavable or steam sterilizable. 6-W{IWq?q$}.w;u]pw8v0 2oxZtcX*q,NCqZ.\;i; QnBrE/M_[_=o Our customer service and technical service organizations are dedicated to you, our customer. Compare the measured air flow rate to the specified flow value on the Certificate of Quality included with the cassette. Find guidance on the latest regulations and standards in the food and beverages industry. Refer to the Pellicon 2 Cassettes Installation, User, and Maintenance Guide (UG2996EN) or the Pellicon 3 Cassettes Installation and User Guide (AN1065EN00) when performing actual set-up and installation of Pellicon cassettes. Depending on individual removal criteria, the total flush volume can be adjusted. Detailed information on trademarks is available via publicly accessible resources. Training programs and seminars designed for you. \caclt h fcforhgc t`ht `hs h GF^A egc-t`mrb te egc-jmjt` t`c feacluahr wcmn`t. Fully open the retentate (and permeate valve if present) and direct both the retentate and permeate lines back to the tank. Training programs and seminars designed for you. Close the tank outlet valve (V1) and drain valve (V2). Catalogs and product finders. The presence of residual cleaning agents can significantly alter integrity test results. hTn@yLTxo"$@QKI-c#{ZV3>sfcs 0B 9n 1~#rmbhW/~Krsf4%];dG%#'Hn`^;SSD0F842u9peBzK feulmIF`)=XnnH6 :mu~EZW:X]a&b^B2)I]/zRhSXFn5ePi(+"d|meo_m !1JX uc 7 y>$jUE&OE5>)6yG8l@aZ'/$u_?T l1Z7"aOIgBIsh/\I((LdJm:0`rxbY2?sRQ0L&[ aJ2cJ,v? Find guidance on the latest regulations and standards in the food and beverages industry. . Tighten the nuts using the torque wrench and 9/16 in. Industrial Microbiology. Sanitization pressures, flow rates and volumes are identical to those used during Cleaning. Both Pellicon 3 cassettes were challenged with bovine gamma globulin (BGG) solution formulated in 10 mM phosphate buffered saline, pH 7.2. Pellicon cassettes assembled in other holders When Pellicon 2 and 3 cassettes are placed in other holders, the torque requirement to achieve Pellicon 3 Cassettes must be operated while under compression from the holder assembly to ensure that the cassettes will seal properly. There is an initial NWP decline in Pellicon 3 Cassettes after initial exposure to 0.5 N NaOH for Ultracel and 1.0 N NaOH for Biomax. Assemble the TFF system as shown in Figure 2. Stable NWP from run to run is achievable after initial exposure. All hydraulic flex hose connections must be installed from the Hydraulic Power Unit to the hydraulic cylinders. Fill the tank with the flushing solution. Pellicon 3 Cassette with Ultracel 30 kDa Membrane, C screen, 0.11 m This guide provides installation and maintenance procedures for Pellicon 3 cassettes. Suggested retentate pressures are recommended to achieve desired feed flow to permeate flow conversion rate. You have already flagged this document.Thank you, for helping us keep this platform clean.The editors will have a look at it as soon as possible. Biopharma Application Guide Find the products and services for your process. Each Pellicon 3 cassette manufacturing lot is 100% integrity tested during manufacturing to ensure that every filter is integral, robust and within specification. Technical Information. With a continuous motion, turn each nut turn, alternating between the nuts, until the wrench clicks, which will indicate it has reached the set force.